WIJI SUCIATI, NIM. C12019056 (2023) PENETAPAN KADAR FLAVONOID TOTAL PADA EKSTRAK ETANOL, FRAKSI n-HEKSAN, ETILASETAT DAN METANOL DAUN TALAS (Colocasia esculenta L.) MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS. Skripsi thesis, UNIVERSITAS MUHAMMADIYAH GOMBONG.
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Abstract
Background: Flavonoids are secondary metabolites that have many benefits as an alternative treatment. Taro (Colocasia esculenta L.) is a plant that contains flavonoids. Objectives: To determine the total levels of flavonoids contain in extract and fractions of taro leaves (Colocasia esculenta L.) using the Uv-Vis Spectrophotometry method. Methods: This research is experimental using taro leaves (Colocasia esculenta L.). Taro leaves were extracted by maceration using 96% ethanol solvent. The extract was standardized with 2 parameters, namely specific and non-specific. Specific parameters include organoleptic test, water soluble extract content and ethanol soluble extract content. Non-specific parameters include drying shrinkage test, water content test, total ash content test and acid insoluble ash content test. The extract was fractionated with n-hexane, ethyl-acetate and methanol . Extracts and fractions of taro leaves (Colocasia esculenta L.) were tested in tubes and TLC. Determination of total flavonoid content was carried out by Uv-Vis spectrophotometry. Result: Viscous extract, water soluble extraxt content was 8,8%, ethanol soluble extract content was 27,2%. Drying shrinkage 3,4%, moisture content 2,55, total ash content 2,0% and acid insoluble ash content 0,5%. Ethanol extract 96%, ethil-acetat fraction and methanol fraction positively contain flavonoids. Thetotal flavonoid content of the ethanol extract 96%, n-hexane fraction, ethil-acetatce fraction and methanol fraction of each was 10,27 mgQE/g, 0,136 mgQE/g, 8,85 mgQE/g and 8,15 mgQE/g. Conclusion: The highest total flavonoid content was obtained from 96% ethanol extract, which was 10.27 mgQE/g). Recommendation: Tests for the levels of secondary metabolites such as tannins, saponins, alkaloids and steroids were carried out to obtain a more varied comparison of data.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Additional Information: | apt. Laeli Fitriyati, M.Farm; apt. Naelaz Zukhruf W.K., M.Pharm.Sci |
| Uncontrolled Keywords: | total flavonoids; taro leaves; extract; fraction; Uv-Vis spectrophotometry; total flavonoid; daun talas; spektrofotometri Uv-Vis; ekstrak; fraksi |
| Subjects: | S1 Farmasi |
| Divisions: | Prodi Farmasi S1 |
| Depositing User: | Unnamed user with email stikesmuhgombong@yahoo.com |
| Date Deposited: | 01 Dec 2023 03:11 |
| Last Modified: | 01 Dec 2023 03:11 |
| URI: | http://repository.unimugo.ac.id/id/eprint/2975 |
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