CHINTYA RAHMA WIDYA PUTRI, NIM. 2021050008 (2025) VALIDASI METODE ANALISIS PENETAPAN KADAR FLUORIDA (F-) DALAM PASTA GIGI ANAK MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS. Skripsi thesis, UNIVERSITAS MUHAMMADIYAH GOMBONG.

Text CHINTYA RAHMA WIDYA PUTRI NIM. 2021050008_compressed.pdf Download (1MB)

Abstract

Background: Children's toothpaste is a popular product because it is specially formulated with flavors that children like and fluoride content to prevent caries. Excessive fluoride levels can cause fluorosis. SNI states that the maximum permissible fluoride content is 500-1000 ppm. Therefore, an accurate analytical method is needed to measure fluoride levels. Method validation is needed to ensure the method meets the set standards. Objective: This study aims to validate the UV-Vis Spectrophotometric method using SPADNS-zirconyl acid reagent for the determination of free fluoride levels in children's toothpaste. Method: The study was conducted experimentally to analyze fluoride levels in 5 children's toothpaste (PG) samples selected through my best website. The validation carried out includes parameters according to ICH 2022 validation guidelines such as specificity, linearity, accuracy, precision, LOD, and LOQ. Results: The validation results of specificity show that the method used has good specificity to fluoride ions without significant interference from other components in the sample, linearity r value 0.9988, accuracy %recovery value 120.20%, precision %RSD value 1.0332%, LOD LOQ values obtained 5.43 ppm and 16.44 ppm, respectively. The result of the fluoride content of sample PG1 was 1260 ppm, PG2 was 505.49 ppm, PG3 was 705.07 ppm, PG4 was 819.88 ppm, and PG5 was 585.96 ppm. Conclusion: The UV-Vis spectrophotometric method was successfully validated and qualified for the analysis of free fluoride in children's toothpaste. Sample PG1 did not meet the standard fluoride content recommended by SNI. Recommendation: Further research is recommended to develop fluoride analysis methods, as well as evaluate the stability of complex colors to determine the operating time to improve measurement accuracy.

Actions (login required)

View Item